No. Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Learn how we performed a multiplexed, primary human cell-based assay to fluorometrically measure inflammation markers using our SpectraMax microplate readers in this poster. The SpectraMax M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. COVID-19 RESPONSE - We are committed to supporting our scientific community during this pandemic. Learn all about fluorescence detection – what it is, how it works, and the instruments used to measure the fluorescence of a sample. Measure sample depth with no temperature dependency using the patented PathCheck® Sensor technology. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening. Latest Citations: For a complete list, please click here . One and 12 h after the last injection, rats were killed and the lungs collected. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. CRL-1658) Minimum Essential Medium (MEM, Corning Cat. Wistar rats received daily subcutaneous injections of Hcy (0.3–0.6 μmol/g body weight) from the 6th to the 28th days-of-age and the control group received saline. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0056 Rev. SpectraMax M5e Multi-Mode Microplate Reader. All rights reserved. Download High Res Photos. The purpose of this paper was to explore the molecular mechanism of the action of baicalein on PC12 cells and isolated rat brain mitochondria. Vascular manifestations such as myocardial infarction, cerebral thrombosis, hepatic steatosis, and pulmonary embolism are common in this disease and poorly understood. Firstly, we investigated the effects of baicalein on rotenone-induced toxicity in PC12 cells. 10-010) Fetal Bovine Serum 0.05% Trypsin EDTA (Corning Cat. ©2021 Molecular Devices, LLC. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 美谷分子仪器（上海）有限公司保留所有权利。 All Rights Reserved 沪ICP备05056171号-1. ©2021 Molecular Devices, LLC. Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. ©2021 Molecular Devices, LLC. Life sciences technology predictions for 2021, Molecular devices unveils next-generation imaging system with deep learning technology, Spectramax Glo Steady-Luc Reporter Assay Kit, Neurotransmitter Transporters Uptake Assay Kit, SpectraMax Injection cartridge with SmartInject Technology, Adjustable Petri Dish and Microplate Holder, CloneSelect Single-Cell Printer Cartridges, The ImageXpress ® Confocal HT.ai High-Content Imaging System. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. SpectraMax M2 Multi-Mode Microplate Reader, SpectraMax M2E Multi-Mode Microplate Reader, SpectraMax M3 Multi-Mode Microplate Reader, SpectraMax M4 Multi-Mode Microplate Reader, SpectraMax M5 Multi-Mode Microplate Reader, SpectraMax M5E Multi-Mode Microplate Reader, SpectraTest ABS2 Absorbance Validation Plate, SpectraTest FL1 Fluorescence Validation Plate, SpectraTest LM1 Luminescence Validation Plate, Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to…, Cell viability can be assessed by examining parameters such as cell membrane integrity or the…, Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein,…, Learn all about fluorescence detection – what it is, how it works, and the instruments used to…, Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in…, Fluorescent proteins have become enormously popular as tools for monitoring biological events in…, Learn about luminescence detection – what it is, how it works, and the advantages of luminescence…, Genotyping is a process for analyzing genetic differences among individuals by examining their DNA…, Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. The results showed that baicalein suppressed rotenone-induced apoptosis, and inhibited the accumulation of reactive oxidant species, ATP deficiency, mitochondrial membrane potential dissipation, and caspase-3/7 activation in a concentration-dependent manner, indicating that baicalein likely improved mitochondrial function. Providing our customers with innovative bioanalytical solutions for protein and cell biology for over 30 years. Previous pharmacological studies found that baicalein had neuroprotective effects in animal models of Parkinson's disease. This microplate reader features two monochromators allowing users to define their optimal excitation and emissions without the need to change filters. for the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. It automatically normalizes absorbance readings to 1 cm, eliminating the need for standard curves. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. Become 21 CFR Part 11 compliant with SoftMax Pro 7.1.1 GxP Software. To optimize signal detection, use solid white microplates rather than clear-bottom ones. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. No. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These results suggest that baicalein may be a mitochondria-targeted antioxidant and exerts neuroprotective effects on rotenone-induced neurotoxicity. 美谷分子仪器（上海）有限公司保留所有权利。 All Rights Reserved 沪ICP备05056171号-1, Baicalein is one of the major flavonoids obtained from the Scutellaria root. Single nucleotide polymorphisms (SNPs) are one of the most common types of genetic variation, consisting of a single nucleotide mutation at a specific locus. Filterless readers provide consistent results with steady temperature regulation . Learn more, Configurable readers with triple-mode cuvette ports, validation tools, and compliance software. Create your account today to get full access to integrated content and world-class customer service. 50 μL of standard was pipetted into the wells of a white…. SoftMax Pro GxP Software extends data acquisition and analysis solution into regulated laboratories working under GMP, GLP, and 21 CFR Part 11 for secure electronic records. Genotyping is a process for analyzing genetic differences among individuals by examining their DNA sequences. They contain a wide range of high-performance capabilities ideal for life science research & drug discovery screening…, Molecular Devices is a leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical development, and biotherapeutic discovery. The CyQUANT Cell Proliferation…, Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. However, this method is only able to measure down to about…, ATP standards ranging from 1 μM to 1 pM were prepared as a 10-fold serial dilution series in phosphate-buffered saline (PBS). SpectraNet is an intuitive, simple-to-use, self-service customer portal providing a new level of experience available 24/7. Plus; Plus384 * SpectraMax i3 Install Guide* SpectraMax i3* SpectraMax i3x* SpectraMax iD3* SpectraMax iD5* SpectraMax M-series Microplate readers. Reduced glutathione content and glucose 6-phosphate dehydrogenase activity, as well as nitrite levels, were also evaluated. 3D-Culture, Organoids, & Organs-on-Chips 2021, Ananda Devices and Molecular Devices launch strategic collaboration to automate imaging and customize analysis of nervous system on-a-chip, Molecular Devices unveils next-generation imaging system with deep learning technology, Imaging system from Molecular Devices helps Recursion produce the largest publicly available set of human cellular morphological data for COVID-19 therapeutic research, Molecular Devices, LLC. Products : Gemini XPS reader, SpectraMax 190 reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M3 reader, SpectraMax M2/M2e reader, SpectraMax M5/M5e reader. For over 13 years, scientists have relied on the industry standard, the SpectraMax® M Series Multi-Mode Designed to help researchers advance phenotypic screening of: Learn how scientists are advancing discovery with our products and solutions. Learn all about absorbance detection – how it works, how it’s measured, and how it can be used to calculate concentration. We’ll provide…. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se Molecular Devices SpectraMax M5e Multimode Microplate Reader Cambridge ID #: 20644. Green fluorescent protein (GFP) from the jellyfish Aequorea victoria was the…, Cyclic nucleotide phosphodiesterases (PDEs) are a group of enzymes that degrade the phosphodiester bond of cAMP and cGMP, second messengers that are involved in a variety of biological…, HTRF is a versatile technology developed by Cisbio Bioassays for detecting biomolecular interactions1. Extensive suite of validation tools reduces the cost and time of validation by 50% as compared to using multiple platforms to collect and analyze data. Discover More: The ImageXpress ® Confocal HT.ai High-Content Imaging System combines premium hardware technology with IN Carta Image Analysis Software, a self-learning software analysis workflow for increased signal and ultra-fast 3D organoid screening. Our team-oriented corporate culture ensures diversity of thought, perspective, and a strong relationship of trust. SNP genotyping has proven very useful in identifying disease-related mutations in various species, and as a result many techniques for SNP detection have been developed. View and Download Molecular Devices SpectraMax M3 user manual online. SpectraMax® M5 Microplate Reader from Molecular Devices LLC Description The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Normal HDL confers protection against atherosclerosis by inhibiting the…. However, this method is only able to measure down to about 250…. 3860 N First Street San Jose, CA 95134. It combines fluorescence resonance energy transfer (FRET) technology with time-…, Traditional ELISA (enzyme-linked immunosorbent assay) methods using the horseradish peroxidase (HRP) substrate 3,3’,5,5’- tetramethylbenzidine (TMB) often fail to detect low abundance…, Genotyping is a process for analyzing genetic differences among individuals by examining their DNA sequences. This video will give you the basics on setting up your Molecular Devices SpectraMax® M5e Microplate Reader to run Cisbio HTRF Assays. For example, a red cell-impermeant, DNA-binding dye only stains dead or dying cells whose membranes are compromised, while a green live-cell dye only fluoresces when metabolic enzymes are active. Our comprehensive suite of proven compliance solutions for GMP/GLP labs can advance your efforts to quickly and confidently establish a compliant laboratory. Microplate Readers, for their consistent performance, reliability and du…, Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. We use cookies to personalize content and improve your browsing experience. O…. MicroplateReader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5e Multi-ModeMicroplateReaderaredualmonochromator,multi-detectioninstrumentswith atriple-modecuvetteportanda6-wellto384-wellplatereadcapability.
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